First series of NANOTUMOR meetings
“Intravital Imaging Club” – In vivo tracking of tumor progression

Credit: B. Bayarmagnai, postdoc in Gligorijevic lab

Webinar series is over


The most sophisticated in vitro and ex vivo approaches fail to faithfully reproduce the complex environment of physiological and pathophysiological processes that occur in vivo. Hence, a “reality check”, in vivo experiments which complement in vitro or ex vivo findings need to be performed. In this context, intravital microscopy (IVM) is an essential approach since it allows the visualization at high resolution of cellular and molecular events in living organisms. The practice of IVM was initiated centuries ago, but recent developments have rendered this technique critical in every researcher’s technological toolbox. However, IVM expertise is spread across multiple countries and to our knowledge actions to share knowledge, pool resources and transcend borders in this field are missing. Our objective is to bring together the leaders in the field of IVM, in particular those working on tumor biology and its microenvironment. As a result, we expect to increase the crosstalk between researchers, foster collaborations and increase awareness of young researchers about the potential of IVM of tumors, in particular, in the context of tumor growth, metastasis and immune escape. Altogether, we aim to promote interactions between established and young researchers to transfer knowledge across generations in the field of IVM.


  • Bojana Gligorijevic – Cancer Microscopy & Mechanobiology Lab. Temple University, College of Engineering, 1947 N. 12th street, Philadelphia, PA 19122, USA –

  • Danijela Vignjevic – Cell migration and invasion Lab. Institut Curie, 26 rue d'Ulm, 75248 Paris Cedex 05, France –

  • Paul Timpson – Invasion and Metastasis Lab. Garvan Institute, 384 Victoria Street, Darlinghurst, Sydney 2010, Australia –


Administrative contact:

  • Florent Colin, NANOTUMOR consortium project manager ( Tumor Biomechanics Lab U1109, Centre de Recherche en Biomédecine de Strasbourg (CRBS), 1 rue Eugène Boeckel, 67084 Strasbourg, France –

Session 1 – Imaging Tumor Progression
April 2, 2021, 12h00 – 14h00, London Time





Chaired by P. Timpson (AU) & J.G. Goetz (FR)

12h00-12h30: Jacco van Rheenen from the Netherlands Cancer Institute and Oncode Institute (Amsterdam, NL) - Keynote overview of intravital application at multiple stages of cancer progression

12h30-13h00: David Bryant from the Cancer Research Beatson Institute (Glasgow, UK) - 3D-modelling of tumours and high throughput automated imaging in cancer

13h00-13h30: Frank Winkler from the German Cancer Research Center (Heidelberg, DE) - Live imaging of malignant networks fosters novel concepts for brain tumor biology and therapy

13h30-14h00: Round table with speakers and chairmen


Credit: B. Bayarmagnai, postdoc in Gligorijevic lab

Credit: L. Perrin, PhD student in Gligorijevic lab


Jacco van Rheenen

David Bryant

Frank Winkler

Session 1 is over - not recorded

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Session 2 – Tumor Transit and Metastasis
April 16 (Friday), 2021, 12h00 – 14h00, London Time





Chaired by D. Vignjevic (FR) & A. Peixoto (FR)

12h00-12h30: Erik Sahai from the Francis Crick Institute (London, UK)Imaging intra-tumour heterogeneity

12h30-13h00: Jacky G. Goetz from the Centre de Recherche en Biomédecine de Strasbourg (Strasbourg, FR) - Intravascular tracking of tumor metastasis

13h00-13h30: Paul Timpson from the Garvan Institute (Sydney, AU) - FRET-based intravital biosensor imaging: assessing CAF hierarchy in driving metastasis of pancreatic cancer

                                13h30-14h00: Round table with speakers and chairmen

Credit: Luc Mercier, Matthia Karreman & Ignacio Busnelli from Goetz lab


Erik Sahai

Jacky G. Goetz

Paul Timpson

Session 2 is over - Recorded!

Session 3 – Co-option of immune cells in disease progression
April 30 (Friday), 2021, 14h00 – 16h00, London Time

Chaired by B. Gligorijevic (USA) & A. Peixoto (FR)




14h00-14h30: Mikala Egeblad from the Cold Spring Harbor Laboratory (New-York, US) - Keynote, intravital insights into myeloid cell recruitment in cancer

14h30-15h00: Leo Carlin from the Cancer Research Beatson Institute (Glasgow, UK) - Live imaging of immune-cancer kinetics in lung tissue


15h00-15h30: Jean-Philippe Girard from the IPBS-CNRS (Toulouse, FR) - Live imaging of lymphocyte trafficking through high endothelial venules in immunity, inflammation and cancer

15h30-16h00: Round table with speakers and chairmen

Mikala Egeblad

Mikala Egeblad Headshot 2_400by400.jpg

Session 3 is over - Recorded in part

Credit: B. Bayarmagnai, postdoc in Gligorijevic lab

Leo Carlin


Jean-Philippe Girard


Credit: Antonio Peixoto, Scientific Head of Imaging Core Facility

Felicity Davis


Tatyana Chtanova


Session 4 – Assessing Dynamics of Immune Interactions
May 14 (Friday), 2021, 11h00 – 13h00, London Time

Chaired by P. Timpson (AU) & D. Vignjevic (FR)


11h00-11h30: Felicity Davis from the University of Queensland (AU) - Multiscale activity imaging in the mammary gland reveals how oxytocin enables lactation


11h30-12h00: Tatyana Chtanova from Garvan Institute (Sydney, AU) - Visualising Innate Immunity in Cancer

12h00-12h30: Philippe Bousso from the Pasteur Institute (Paris, FR) - Decoding the mode of action of tumor immunotherapies using intravital imaging

                                12h30-13h00: Round table with speakers and chairmen

Philippe Bousso


Session 4 is over - Recorded in part

IVM LN1.tif

Session 5 – Tumor ECM Interactions and Communication

May 28 (Friday), 2021, 16h00 – 18h00, London Time

Chaired by B. Gligorijevic (USA) & D. Vignjevic  (FR)



16h00-16h30: Guillaume Jacquemin from the Institut Curie (Paris, FR) & Bethan Lloyd-Lewis from the University of Bristol (UK) - Longitudinal intravital imaging of cellular dynamics in developing tissues and tumours

16h30-17h00: Chris Madsen from Lund University (Lund, SE) - ECM imaging of the tumour niches

17h00-17h30: Kenneth Hu from the University of California San Francisco (US) - Alignment of scRNA-Seq and imaging on live tumor sections highlights myeloid subpopulations with contrasting spatial localizations and roles in the TME.

17h30-18h00: Round table 

Bethan Lloyd-Lewis_picture_crop.tiff

Bethan Lloyd-Lewis

Guillaume Jacquemin

Session 5 is over - Recorded in part

Credit: B. Bayarmagnai, postdoc in Gligorijevic lab

Chris Madsen


Kenneth Hu


Credit: Vincent Hyenne, reseacher in Goetz lab

Session 6 – Imaging the Niche – part 1/2

June 11 (Friday), 2021, 14h00 – 16h00, London Time

Chaired by A. Peixoto (FR) & J. G. Goetz (FR)




14h00-14h30: Franck Debardieux the Institute of Neurosciences, Timone (Marseille FR) - Multi-photon based long- and short-term imaging of immune cell recruitment

14h30-15h00: Giorgio Seano from the Institut Curie (Orsay, FR) - Intravital and preclinical vasculature imaging in the brain

15h00-15h30: Bojana Gligorijevic from Temple University (Philadelphia, US) - Real-time imaging of invadopodia and the perivascular niche

                           15h30-16h00: Round table with speakers and chairmen


Franck Debardieux

Giorgio Seano

Bojana Gligorijevic


Session 6 is over - Fully recorded!

Session 7 – Imaging the Niche – part 2/2
June 25 (Friday), 2021, 14h00 – 16h00, London Time

Chaired by D. Vignjevic (FR) & J. G. Goetz (FR)




14h00-14h30: Roberto Weigert from the National Cancer Institute, NIH (Bethesda, MD, USA) - Longitudinal Imaging of tumor progression at cellular and subcellular level in head and neck cancer

14h30-15h00: Anoek Zomer from Ludwig Institute for Cancer Research, University of Lausanne (Lausanne, CH) - Intravital imaging microenvironmental regulation of tumor progression and therapeutic response

15h00-15h30: Kendelle Murphy from the Garvan Institute (Sydney, AU) - Intravital imaging technology guides FAK priming in pancreatic cancer precision medicine according to Merlin status.

15h30-16h00: Round table with speakers and chairmen

Roberto Weigert


Session 7 is over

Credit: L. Perrin, PhD student in Gligorijevic lab

Anoek Zomer


Kendelle Murphy